I always get a chuckle over Coop's target after his turn on the firing range-- 'I put two through each eye and one through each nostril.'

I also love Cathering bitch-slapping Ben Horne in his office over the poker chip from One Eyed Jack's, their subsequent clinch, and then Ben winds up the whole thing by offering Catherine a breath mint. Too funny!

Also, Andy's confession to Lucy that he applied as a donor at the Tacoma Sperm Bank 'because it's my civic duty, and besides, I like whales...'

'HOO-HOO, HOO-HOO-HOO-OO-OO...'

                                   --Various Owls in random trees, in and around Twin Peaks.

* he makes out with Catherine and then offers her a breath mint

* Jerry really thinks he killed Laura

..."Is this thing ON?!"....   gets me every time too!

MAJ Briggs slapping the cigarette out of Bobby's mouth, landing in the meatloaf.  Priceless!!

Any moment with Albert

Ben and Jerry eating the Sandwiches at the dinner table.

Andy getting hit in the head by the 4x8.

Coop and the Llama

Peter telling Coop and Harry there's a fish in the perculator, sorry

Cooper and the llama, Ben offering Catherine the mint, Albert's insults (loving Albert, all the way) and the way Margaret says "Shut your eyes and you'll burst into flames" and Harry goes "Thanks Margaret!".

Honourable mention to the Catherine / Leland "despair dance", the whole scene with Audrey and the Norwegians in the pilot, and the bit where the morgue attendant mis-hears Coop and says "Jim"  

Jack and Catherine's only scene together.

And then Andy dances with Denise and looks... really really happy about it.

Thanks Angel, I forgot how funny it is to see Jack and Catherine together. Actually they are in the final episode as well.

Where's my truck?? 
 

"What? Did your life pass before your eyes? Cuppa tea, cuppa tea, almost got shagged, cuppa tea..."

Ben:  "We've laid in a gala reception for your fair-haired boys tonight.  All of Twin Peaks' best and brightest."

Jerry: "We're holding it in a phone booth?"

Heh. Thanks, Rob, Getting rusty in my old age.  It was the first scene they were in together since their marriage dissolved, then.

If I ever get arrested, I will straighten out my lapels and try to go make a sandwich. Ben is a legend. A legend.

Oh, man. I forgot about the way he straightens his lapels.  
 

"What? Did your life pass before your eyes? Cuppa tea, cuppa tea, almost got shagged, cuppa tea..."

Just got a chuckle out of the room service guy shuffling across the floor in the black lodge saying "Coffee?  Coffee?  Coffee?"

I really like that Chiller is showing Twin Peaks.

Striving for the narrow gate.  

Ben:  "We've laid in a gala reception for your fair-haired boys tonight.  All of Twin Peaks' best and brightest."

Jerry: "We're holding it in a phone booth?"

The "OK, let's humor the crazy lady" look on Shelly's face when Nadine asks if Shelly goes to school with her.

"What? Did your life pass before your eyes? Cuppa tea, cuppa tea, almost got shagged, cuppa tea..."

Another good one from the pilot -

Jacoby - "Gary Cooper?"

Ben:  "We've laid in a gala reception for your fair-haired boys tonight.  All of Twin Peaks' best and brightest."

Jerry: "We're holding it in a phone booth?"

Followed shortly thereafter by Cooper's surreptitious "That guy's a psychiatrist?"